FIGURE 9.

Effect of anion-channel inhibitor on VacA-induced LC3-II generation. AZ-521 (a) or AGS (b) cells were pretreated with 100 μm NPPB or DIDS for 30 min at 37 °C and then incubated with 120 nm heat-inactivated (IA) or wild-type VacA (A) for 4 h at 37 °C. Cell lysates were subjected to immunoblotting (IB) with anti-LC3B antibody or anti-α-tubulin antibody as a loading control. Quantification of VacA-induced LC3-II levels in the cells was performed by densitometry (bottom panel). Data are mean ± S.D. of values from two independent experiments.