FIGURE 3.
Effect of ErbB2 on EGF receptor dimerization. A, CHO cells stably expressing ΔC-EGFR-NLuc and ΔC-EGFR-CLuc were stimulated with the indicated concentrations of EGF and receptor dimerization was monitored via luciferase activity. B, CHO cells stably expressing ΔC-ErbB2-NLuc and ΔC-EGFR-CLuc were stimulated with the indicated concentrations of EGF and receptor dimerization was monitored via luciferase activity. C, the data in panels A and B were fit to a single exponential association model using Prism 4.0 and the derived kobs values were plotted against the [EGF]. These data were then fit to a sigmoidal dose-response curve using Prism 4.0. The fitted EC50 values are reported in the text.