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Cmc1 is an in vitro substrate of Erv1. A, time course of oxidation of reduced Cmc1 by Erv1. B, effect of Mia40 in Cmc1 partially oxidized by Erv1. Reduced Cmc1 was mixed with Erv1 for 4 h. Next, Erv1 was removed by His tag affinity cobalt beads. The Erv1-free sample was then incubated with or without Mia40 for 2 h. C, titration of Erv1 with Cmc1 held constant at 1 μm. In A–C, the samples were treated as in Fig. 1.
