FIGURE 4.

ATOM single channels respond to a genuine mitochondrial presequence from T. brucei in a side-specific manner. A, current traces of a single channel depict the increased frequency of conductance changes upon the addition of 2 μm DLDH peptide. B, the gating frequency in the presence of increasing peptide concentrations was normalized with respect to the gating frequency in the absence of peptide (n ≥ 2; 4 single channels). In the presence of 5 μm peptide, the gating frequency could only be measured for one channel. Buffer conditions were symmetric with 250 mm KCl, 10 mm MOPS/Tris, pH 7, in cis and trans. Error bars indicate S.D.