TABLE 1.

Effects of mutations on the IgE-CD23 interaction

IgE-Fc mutation	KD	ΔΔG	derCD23 mutation	KD	ΔΔG
	μm	kJ mol−1		μm	kJ mol−1
Wild type	2.3		Wild type	2.3
D409A	26.3	+6.0	D227A	30.9	+6.4
E412A	24.2	+5.8	E257A	26.7	+6.1
R376A	19.7	+5.3	R224A	26.2	+6.0
K380A	13.3	+4.3	R188A	25.0	+5.9
K435A	5.0	+1.9	Y189A	15.6	+4.7
K352A	3.8	+1.2	K276A	10.9	+3.9
R351A	2.6	+0.3	L226A	6.6	+2.6
D347A	2.5	+0.2	D236A	5.8	+2.3
P439A	2.5	+0.2	D192A	4.3	+1.6
Q535A	2.5	+0.2	E265A	2.5	+0.2
Q538A	2.4	+0.1