FIGURE 1.

Renal expression of factor H. A, factor H (in red) is present in interstitium of normal rat renal tissue. B and C, factor H is present in interstitium and luminal side of tubular cells (arrowheads) in protein overload (B) and adriamycin nephropathy (C). D–G, factor H (in red) is absent in luminal side of the tubuli in normal rat tissue. Phalloidin-FITC (in green) stains the brush border of the proximal tubuli. H–K, presence of factor H (in red) on top of phalloidin-FITC (in green) shows the luminal presence of factor H (white asterisks) in protein overload proteinuric rat renal tissue. Each scale bar shows 50 μm. The nuclei are stained with DAPI in blue.