FIGURE 3.
Comparison of agonist-induced responses in CB1R knock-out and wild type mice. Vasodilations induced by the CB1R agonist WIN55212 (1 μm) and by the endothelial vasorelaxant Ach (10 μm) as well as the effects of CB1R inhibition by the O2050 (1 μm) are shown in saphenous arteries of wild type (A, +/+) and of CB1R-deficient (B, −/−) mice (n = 4–6). C and D, shown is modulation of Ang II concentration-response curves by O2050 (1 μm) in the two mice strains (n = 4–5). Mean ± S.E. values are shown. The asterisk indicates significant effect of agonist or antagonist, p < 0.05. Data obtained with curve-fitting method showed that in wild type mice Emax values were 20.4 ± 3.7 and 40.3 ± 6.9% (p < 0.05, n = 4–5), and log EC50 values were −9.1 ± 0.3 and −8.8 ± 0.4 m in the absence and in presence of O2050, respectively. In CB1R-KO mice Emax values were 28.7 ± 7.1 and 29.4 ± 6.4% in the absence and presence of O2050, respectively. Changes in EC50 values were not significant.