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GC-MS analysis of BR metabolites produced by heterologously expressed CYP90A1/CPD. Selected ion chromatograms of the reaction products were obtained with 6-deoxoCT (fragment ion for detection at m/z 97 and 187) (A), 22-OHCR (fragment ion for detection at m/z 97 and 187) (B), 6-deoxoTE (fragment ion for detection at m/z 155 and 231) (C), and 22,23-OHCR (fragment ion for detection at m/z 124 and 229) (D) as substrates. C-3 oxidation of BR biosynthetic intermediates by CYP90A1/CPD (E).
