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Relative activities of CYP90A1/CPD with 6-deoxoCT, 22-OHCR, 6-deoxoTE, and 22,23-diOHCR substrates. CYP90A1/CPD assay mixtures were supplemented with BRs (to 20 μm), incubated at 30 °C for 20 min, and the reaction products were then analyzed by GC-MS. The activity obtained with 22-OHCR substrate (100%) corresponds to 2.27 units (defined as pmol of product/min/pmol of P450). Values were determined in four replicate measurements; error bars indicate standard deviation.
