TABLE 4.

GC-MS data of products obtained in DET2 enzymatic assays

Substrate	Product	RT	Characteristic ions, m/z (relative intensity percentage)
		min
4-en-3-one	3-one	9.16	400 (12), 385 (7), 317 (2), 231 (100)
	Synthesized	9.20	400 (11), 385 (7), 317 (1), 231 (100)
22-OH-4-en-3-one	22-OH-3-one	11.70	398 (6), 302 (35), 271 (40), 231 (100)
	Synthesized	11.67	398 (6), 302 (30), 271 (43), 231 (100)
22,23-diOH-4-en-3-one	6-Deoxo3DT	11.05	456 (7), 283 (6), 231 (100), 155 (59)
	Synthesized	11.03	456 (10), 283 (8), 231 (100), 155 (56)