FIGURE 5.

G478C mutation alters voltage-dependent movements of C260-TMRM and C483-TMRM in absence of organic cations, whereas MTSET⁺ modification of Cys-478 alters voltage-dependent movement of C380-TMRM. rOct1(10ΔC, P260C), rOct1(10ΔC, P260C,G478C), rOct1(10ΔC,F380C), rOct1(10ΔC,F380C,G478C), rOct1(10ΔC,F483C), and rOct1(10ΔC,F483C,G478C) were expressed in oocytes. All oocytes were labeled with TMRM. In part of the oocytes, voltage-dependent fluorescence changes were measured after TMRM labeling. The fluorescence measurements with rOct1(10ΔC,C260-TMRM), rOct1(10ΔC,C380-TMRM), and rOct1(10ΔC,C483-TMRM) are indicated as lines (for individual measurements see Fig. 2). Part of the oocytes expressing rOct1(10ΔC,P260C,G478C), rOct1(10ΔC,F380C,G478C), and rOct1(10ΔC,F483C,G478C) was additionally modified with MTSET⁺, and voltage-dependent fluorescence changes were analyzed. Mean values ± S.E. of 4–6 oocytes from three different oocyte batches are indicated.