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. 2012 Jul 16;287(37):31596–31607. doi: 10.1074/jbc.M112.383299

TABLE 2.

Binding affinities (KD) of residues that underwent signal fast exchange during the titration of 15N-labeled Ca2+/CaM with Cpep

R2 values describe the goodness of fit of titration data with R2 of 1, indicating that the experimental values matched with the predicted values using a 1:1 model.

Phe16 Val55 Ala57 Thr70 Arg74 Lys115 Leu116 Val121 Ile130
KD ± S.D. (μm) 47.3 ± 8.1 59.2 ± 16.2 4.9 ± 2.3 28.4 ± 9.6 24.4 ± 5.6 9.3 ± 5.2 5.3 ± 2.3 9.4 ± 4.4 4.6 ± 2.2
R2 0.997 0.994 0.990 0.990 0.995 0.983 0.990 0.986 0.990