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. 2012 Jun 14;4(3):398–402. doi: 10.3892/ol.2012.760

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Induction of multilineage mescenchymal cell differentiation. (A-D) Adipogenesis. In the control group (without adipogenic differentiation), (A) no bright lipid droplets/vacuoles or (B) red stained cells were observed. Following adipogenic differentiation, (C) bright lipid droplets/vacuoles were clearly observed without staining and (D) cells demonstrated a positive reaction using Oil-Red-O staining. (E-H) Osteogenesis. (E and F) In the control group (without osteogenic differentiation), (F) ALP staining revealed only slight positivity. (G and H) Following osteogenic differentiation, (G) cells revealed a cuboidal morphology and (H) ALP staining revealed strong positivity. ALP, alkaline phosphatase.

Induction of multilineage mescenchymal cell differentiation. (A-D) Adipogenesis. In the control group (without adipogenic differentiation), (A) no bright lipid droplets/vacuoles or (B) red stained cells were observed. Following adipogenic differentiation, (C) bright lipid droplets/vacuoles were clearly observed without staining and (D) cells demonstrated a positive reaction using Oil-Red-O staining. (E-H) Osteogenesis. (E and F) In the control group (without osteogenic differentiation), (F) ALP staining revealed only slight positivity. (G and H) Following osteogenic differentiation, (G) cells revealed a cuboidal morphology and (H) ALP staining revealed strong positivity. ALP, alkaline phosphatase.