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. Author manuscript; available in PMC: 2012 Sep 11.
Published in final edited form as: Science. 2012 May 10;336(6086):1325–1329. doi: 10.1126/science.1222195

Fig. 4. Similar catabolic preferences for saccharides may determine the competing ability of commensal bacteria with the enteric pathogen.

Fig. 4

A, GF mice were infected with WT C. rodentium (Cr). At day 21 post infection, E. coli (Ec) or B. thetaiotaomicron (Bt) or B. vulgatus (Bv) were inoculated. As a second inoculation, mixture of B. thetaiotaomicron and B. vulgatus was inoculated into the E. coli harboring group, and E. coli was inoculated into the Bacteroides harboring group, respectively. Pathogen load was determined in feces on the indicated days after inoculation of commensal bacteria. Dots represent individual mice from 2 independent experiments. B, Total Enterobacteria (C. rodentium and E. coli) and Bacteroides culture in feces at day 0 and day 14 (first inoculation in panel A). Data are given as mean ± SD (n=4). Results are representative of at least 2 experiments. C, Number of C. rodentium (kanamycin resistant) in the total Enterobacteria (kanamycin sensitive) are indicated as a percentage. Data are given as mean ± SD (n=4). Results are representative of at least 2 experiments. D, Carbohydrate catabolic profiles of C. rodentium and commensal bacteria strains. Robust growth under supplementation of monosaccharides (MSs) or polysaccharides (PSs) indicated as red, and no growth indicated as yellow. Raw data is provided in Table S1. E, GF mice were infected with WT C. rodentium (Cr). At day 21 post infection, B. thetaiotaomicron (Bt) was inoculated. On day 7 post-colonization with Bt, the mice were divided into two groups that were fed a conventional maintenance diet (C) or a simple sugar diet (ss). Pathogen load was determined in feces on day 3 after diet switching. Results are given as mean ± SD of individual mice (initially n=10, and divided into 2 groups n=5 each). Results are representative of 2 experiments. F, C. rodentium mono-associated GF mice (day 21) were fed with a simple sugar diet (ssDiet) for 7 days. Pathogen load was compared before and after switching to ssDiet from conventional diet (cDiet). Dots represent individual mice and representative of 3 independent experiments **; p<0.01. N.S., denotes not significant by Mann-Whitney U test.