Figure 6. Experimental Characterisation of Segmentations.

Randomly sampled E state segments (see table 3) from the K562 segmentation were cloned for mouse- and fish-based transgenic enhancer assays. Panel A shows a representative LacZ-stained transgenic e11.5 mouse embryo obtained with construct hs2065 (EN167, chr10:46,052,882-46,055,670, GRCh37). Highly reproducible staining in the blood vessels was observed in 9 out of 9 embryos resulting from independent transgenic integration events. Panel B shows a representative green fluorescent protein reporter transgenic medaka fish obtained from a construct with a basal hsp70 promoter on meganuclease based transfection. Reproducible transgenic expression in the circulating nucleated blood cells and the endothelial cell walls was seen in 81 out of 100 transgenic tests of this construct.