Figure 5.

ING4-induced expression changes in some of the genes involved in the cell cycle and apoptosis. (A) Real-time PCR analysis showed that compared with control group cells, the transcriptional expressions of p21 and bax were markedly upregulated in the stably transfected ING4 cells (P<0.05), while the transcriptional expression of p53 was not significantly different to control groups. All data represent the mean of three independent experiments. (B) The expression of p21, bax and p53 proteins in MCF-7 cells. The protein lysates were analyzed by western blot analysis with anti-p21, anti-bax and anti-p53 antibodies. The mRNA and protein expression levels were similar in all groups of cells. P21, bax and p53 levels were normalized against β-actin expression.