Table 3.
Genes Selected for Taqman RT-PCR
| Gene Symbol | Gene Name | Key | Targeted by |
|---|---|---|---|
|
CCND1 |
Cyclin D1 |
1,3 |
AHR/ARNT, PAX9, RAR/RXR |
|
ERBB3 |
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 |
2,3 |
OLF-1 |
|
FOXO3 |
Forkhead box protein O3 |
1,3 |
RAR/RXR |
|
NR3C1 |
nuclear receptor sub family 3 group C member 1 |
2,3 |
AHR/ARNT, PAX3 |
|
RB1CC1 |
RB1 inducible coiled coil protein 1 |
3 |
ZNF143 |
| ALDH3A2 |
aldehyde dehydrogenase 3 family member 2a |
2+ |
|
| CDKN1B |
cyclin dependent kindase inhibitor 1B |
2+ |
|
| PIK3C3 |
phosphoinositide 3 kinase class 3 |
2+ |
|
| AKT1 |
v-akt murine thymoma viral oncogene homolog 1 |
3 |
RAR/RXR |
| BID |
BH3 interacting domain |
2 |
|
| E2F3 |
E2F transctription factor 3 |
1,3 |
AHR/ARNT, OLF-1, PAX9, ZNF143 |
| eIF4E |
eukaryotic translation initiation factor 4e |
3 |
PAX3, RAR/RXR |
| FKBP4 |
Fk506 binding protein 4 |
2,3 |
ZNF143 |
| MAPK9 |
mitogne-activated protein kinase 9 |
2 |
|
| PARP2 |
poly (ADP-ribose) polymerase 2 |
2 |
|
| PSMD13 |
proteasome 26 S subunit non-ATPase 13 |
2 |
|
| SLC29A1 |
solute carrier family 29 member 1 |
2 |
|
| TFPT |
TCF3 (E2A) fusion partner |
2,3 |
ZNF143 |
| CBFA2T2 | core-binding factor, runt domain, alpha subunit 2; translocated to, 2 | 2 |
1 denotes a highly differentially regulated gene (±2 fold across all 6 comparisons) 2 denotes a gene that was known to be associated with cancer, 3 denotes a gene was predicted to be targeted by one or more of the transcription factors. Those in bold were found to be consistently dysregulated in response to lapatinib in all 6 of the cell lines. + denotes that the gene was found to be differentially expressed in BT474 and SKBR3 cell lines but not in the additional four cell lines also tested