Table 1.
Bacterial strains and plasmids used in this study
| Plasmid or strain | Relevant genotype | Reference or source |
|---|---|---|
| Plasmids |
|
|
| pET-30a(+) |
pBR322 ori lacI T7lac Kanr |
Novagen |
| pACYCDuet-1 |
P15A ori lacI T7lac Cmr |
Novagen |
| pCOLADuet-1 |
ColA ori lacI T7lac Kanr |
Novagen |
| pBAD/Myc-HisA |
pBR322 ori araBAD Ampr |
Invitrogen |
| pZh05 |
pACYCDuet-1 harboring FAR from Jojoba and fadD from E. coli |
This study |
| pZh07 |
pACYCDuet-1 harboring acr1 from A. baylyi and fadD from E. coli |
This study |
| pZh08 |
pACYCDuet-1 harboring CER4 from A. thaliana and fadD from E. coli |
This study |
| pZh09 |
pACYCDuet-1 harboring FAR from Jojoba and yngl from B. subtilis |
This study |
| pZh10 |
pACYCDuet-1 harboring FAR from Jojoba and FAA2 from S. cerevisiae |
This study |
| pZh11 |
pACYCDuet-1 harboring acr1 from A. baylyi and yngl from B. subtilis |
This study |
| pZh12 |
pACYCDuet-1 harboring acr1 from A. baylyi and FAA2 from S. cerevisiae |
This study |
| pZh17 |
pCOLADuet-1 harboring acr1 from A. baylyi and fadD from E. coli |
This study |
| pZh18 |
pCOLADuet-1 harboring acr1 from A. baylyi, fadD from E. coli and BTE from U. californica |
This study |
| pZh27 |
pBAD/Myc-HisA harboring acr1 from A. baylyi and fadD from E. coli |
This study |
| pYN2 |
pET-30a(+) harboring BTE from U. californica |
26 |
| pLL4 |
pCOLADuet-1 harboring ‘tesA from E. coli |
This study |
| pYN12 |
pCOLADuet-1 harboring BTE from U. californica |
This study |
| pXW2 |
pACYCDuet-1 harboring BTE from U. californica |
This study |
| pYN22 |
pBAD/Myc-HisA harboring BTE from U. californica |
This study |
| Strains |
|
|
| DH5α |
F-,φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR,recA1, endA1, hsdR17(rk-,mk+), phoA, supE44, λ-,thi-1, gyrA96, relA1 |
Takara |
| BL21 (DE3) |
F-ompT gal dcm lon hsdSB(rB-mB-) λ(DE3) |
Invitrogen |
| Zh05 |
E. coli BL21 (DE3) bearing pZh05 |
This study |
| Zh07 |
E. coli BL21 (DE3) bearing pZh07 |
This study |
| Zh08 |
E. coli BL21 (DE3) bearing pZh08 |
This study |
| Zh072 |
E. coli BL21 (DE3) bearing pZh07 and pYN2 |
This study |
| Zh112 |
E. coli BL21 (DE3) bearing pZh11 and pYN2 |
This study |
| Zh122 |
E. coli BL21 (DE3) bearing pZh12 and pYN2 |
This study |
| Zh054 |
E. coli BL21 (DE3) bearing pZh05 and pLL4 |
This study |
| Zh094 |
E. coli BL21 (DE3) bearing pZh09 and pLL4 |
This study |
| Zh104 |
E. coli BL21 (DE3) bearing pZh10 and pLL4 |
This study |
| Zh0712 |
E. coli BL21 (DE3) bearing pZh07 and pYN12 |
This study |
| Zh172 |
E. coli BL21 (DE3) bearing pZh17 and pXW2 |
This study |
| Zh18 |
E. coli BL21 (DE3) bearing pZh18 |
This study |
| Zh0722 |
E. coli BL21 (DE3) bearing pZh07 and pYN22 |
This study |
| Zh1722 |
E. coli BL21 (DE3) bearing pZh17 and pYN22 |
This study |
| Zh272 |
E. coli BL21 (DE3) bearing pZh27 and pXW2 |
This study |
| Zh2712 | E. coli BL21 (DE3) bearing pZh27 and pYN12 | This study |