Figure 2.

CI turning on P_RM. (a) A two plasmid system was used to test the ability of P_RM activation by CI. This circuit consists of an IPTG inducible plasmid that expresses CI and a reporter plasmid expressing GFP by activation of P_RM. Two different ssrA tags were added to the CI protein (ec-ssrA, mf-ssrA) to test the effect of ssrA tags on P_RM activation. (b) Fluorescence measurements were normalized to OD₆₀₀ to account for cell density changes. Strains were cultured in various concentration of IPTG to test for P_RM activation. Error bars denote the measurement range (highest and lowest value). All data points were taken with three biological replicates.