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PLtetO-1activity in the bistable switch. PLtetO-1 activity was measured using the PLtetO-1-GFP reporter. Blue curves represent samples cultured overnight in IPTG. No hysteresis was measured in (a) SW3, (b) SW4, or (c) SW7. Each data point was measured with three biological replicates. Error bars represent the data range (highest and lowest value).
