Table 1.
List of Plasmids (accession numbers are listed in the supplemental section S.5)
| Name | Description | Marker | Origin |
|---|---|---|---|
| PRM-GFP |
GFP reporter expressed from PRM |
Amp |
p15A |
| PLtetO-1-GFP |
GFP reporter expressed from PLtetO-1 |
Amp |
p15A |
| PLlacO-1-CIwt |
IPTG inducible CI-wt |
Cm |
ColE1 |
| PLlacO-1-CILVA |
IPTG inducible CI-ec-ssrA |
Cm |
ColE1 |
| PLlacO-1-CImf |
IPTG inducible CI-mf-ssrA |
Cm |
ColE1 |
| RFP1 |
PLtetO-1 mf-lon (trunc) mCherry with RBS1 |
Amp |
p15A |
| RFP2 |
PLtetO-1 mf-lon (trunc) mCherry with RBS2 |
Amp |
p15A |
| RFP3 |
PLtetO-1 mf-lon (trunc) mCherry with RBS3 |
Amp |
p15A |
| RFP4 |
PLtetO-1 mf-lon (trunc) mCherry with RBS4 |
Amp |
p15A |
| RFP5 |
PLtetO-1 mf-lon (trunc) mCherry with RBS5 |
Amp |
p15A |
| RFP6 |
PLtetO-1 mf-lon (trunc) mCherry with RBS6 |
Amp |
p15A |
| RFP7 |
PLtetO-1 mf-lon (trunc) mCherry with RBS7 |
Amp |
p15A |
| SW1 |
Full switch with 5-UTR1 |
Kan |
pSC101 |
| SW2 |
Full switch with 5-UTR2 |
Kan |
pSC101 |
| SW3 |
Full switch with 5-UTR3 |
Kan |
pSC101 |
| SW4 |
Full switch with 5-UTR4 |
Kan |
pSC101 |
| SW5 |
Full switch with 5-UTR5 |
Kan |
pSC101 |
| SW6 |
Full switch with 5-UTR6 |
Kan |
pSC101 |
| SW7 |
Full switch with 5-UTR7 |
Kan |
pSC101 |
| PRM-CIwt-TetR |
CI-wt and TetR expressed from PRM |
Kan |
pSC101 |
| PRM-CILVA-TetR |
CI-ec-ssrA and TetR expressed from PRM |
Kan |
pSC101 |
| PRM-CImf-TetR |
CI-mf-ssrA and TetR expressed from PRM |
Kan |
pSC101 |
| PLlacO-1-mfLon | IPTG inducible mf-Lon | Cm | ColE1 |