Figure 2.

Immune adaptation in response to SL3261 infection. (A-C) Cytokine production in response to systemic challenge with Salmonella typhimurium SL3261. Levels of protein expression of inflammatory cytokines in serum (A), spleen (B), and brain (C) in naïve non-infected or SL3261-infected mice. Cytokine levels were measured at 1, 7, 14, and 21 days after the infection with 10⁶ cfu SL3261 and compared to cytokine levels in naïve mice. (D) Changes in burrowing behaviour, body weight and serum endotoxin levels upon SL3261 infection. Behaviour experiments were carried out with n = 5 per time point. Error bars represent SEM from five animals per time point (P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) versus naïve control). This experiment was repeated two times with similar results.