Table 1. Expression of type I interferon and IL-6 in AEC co-cultured with MDDC.
AEC | AEC(GM-CSF, IL-4) | AEC+MDDC(GM-CSF, IL-4) | |
IFNα2 mRNA | 1.5±0.01 | 3.8±0.07 * | 10.3±2.2 * |
IFNβ mRNA | 1.3±0.005 | 4.1±0.04 * | 13.6±4.3 * |
IL-6 (pg/ml) | <100 | <100 | 26650±2200 |
AEC were cultured alone, in the presence or absence of GM-CSF + IL-4, or with MDDC. Following 5 days of culture RNA was extracted from sorted AEC and relative expression IFNα2 and IFNβ. *indicates p<0.05 relative to AEC alone (N = 6). IFNα protein was undetectable in culture supernatants. IL-6 protein data are from two experiments.