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The replications of AIV (F98 or H5N1) in ECE after inoculated first with NDV (Lasota or F48E8) were measured by using the Real-time PCR. Supercript 12 h represtents 12 hours interval between two viruses. Superscript 24 hours interval between two viruses. Bars represents the standard deviations for two experiments carried out on three eggs. Statistical significance between the experimental group and control group was determined by the student’s t-test. *p<0.05, **p<0.01, ***p<0.001.
