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. 2012 Sep 11;7(9):e44697. doi: 10.1371/journal.pone.0044697

Figure 4. Schematic representation of the pCAMBIA2301::Scdr1 construct and PCR confirmation of plant transgene content.

Figure 4

(A) The Scdr1 coding region was cloned between the constitutive CaMV 35S promoter (P35S) and the NOS polyadenylation signal (Nos-t) using pCambia2301 as the backbone. The nptII (kanamycin resistance) gene is also driven by the p35S promoter. LB and RB correspond to the left and right borders of the T-DNA, respectively. The positions of some restriction sites are indicated. (B) Expression of Scdr1 in WT and three T3-generation transgenic lines. Total RNA was extracted from two-week-old seedlings and then analyzed using semi-quantitative RT-PCR. The actin gene was used as an internal standard. (C) Densitometric analysis of the semi-quantitative RT-PCR.