Figure 5. MED suppresses LPS-induced MAPK activation.
A, B. RAW264.7 cells (A) and primary peritoneal macrophages (B) were treated with LPS (100 ng/ml) together with various doses of MED for 20 min. The phosphorylation of MAPKs including JNK, ERK1/2 and p38 was determined by Western blotting analysis. C. Effect of MED on phosphorylation of TAK1 induced by LPS in RAW264.7 cells. Cells were treated with LPS (100 ng/ml) together with various doses of MED for 10 min.