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. 2012 Sep 11;7(9):e43456. doi: 10.1371/journal.pone.0043456

Figure 2. Effect of 5-aza-dC treatment on SFN and TKTL1 mRNA expression in 5-aza-dC treated pancreatic CAFs and control fibroblasts, and bisulfite sequencing analysis of TKTL1.

Figure 2

(A) Affymetrix exon array analysis of SFN mRNA expression in five pancreatic CAFs before (green) and after (red) 5-aza-dC treatment. (B) and (C) Quantitative RT-PCR analysis of SFN and TKTL1 mRNA expression relative to GAPDH mRNA in pancreatic fibroblast cultures before (blue bars) and after (red bars) 5-aza-dC treatment. Each assay was performed in triplicate. Data are means of three independent experiments; bars are SD values. (D) Top: TKTL1 gene structure and distribution of CpG dinucleotides. Short vertical bars represent CpG sites. Arrow points to transcriptional start site. Below: Bisulfite genomic sequencing analysis in pancreatic CAFs and control fibroblasts. Open circles represent unmethylated CpG sites, solid black circles methylated CpG sites, and hatched circles partially methylated CpG sites. (E) Bisulfite sequencing chromatograms of the TKTL1 promoter in a pancreatic CAF (CAF19) and control fibroblast lines (HPNE and SC2). Arrows point to cytosine residues.