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. 2012 Sep 11;7(9):e43330. doi: 10.1371/journal.pone.0043330

Figure 1. Organization and expression of the xTNIK and xMINK kinases.

Figure 1

A) Diagrammatic representation of the domain structure of xTNIK and xMINK. The N-terminal Kinase and C-terminal CNH domains are indicated as well as the variably spliced regions within the Central domain of xTNIK. Expression constructs of xTNIK used throughout the manuscript were derived from a cDNA containing all four variable regions. B) In situ wholemount hybridization for xTNIK mRNA. “dbl” dorsal blastopore lip. C) Phenotypic effects of xTNIK and xMINK knockdown. Morpholinos against xTNIK (MoT#1 and -#2) and xMINK (MoM) mRNAs or control Morpholinos (Ctrl Mo) were injected singly and in combinations into the two dorsal blastomeres of four cell embryos and embryos allowed to develop until stage 39–40. Morpholino amounts injected per embryo are indicated as are the fractions of embryos showing the indicated phenotypes. D) Rescue of knockdown phenotypes. Morpholinos MoT and MoM were injected alone or coinjected with the indicated amounts of mRNA encoding the full-length xTNIK or xMINK.