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. 2012 Sep 11;7(9):e43176. doi: 10.1371/journal.pone.0043176

Figure 3. The RT-PCR results of the re-annotated coding ORFs.

Figure 3

(A) The cDNAs of the early log stage cells, the PC-1 (322 bp), PC-2 (291 bp), PC-6 (341 bp), PC-7 (235 bp), PC-8 (251 bp), PC-9 (325 bp), PC-11 (400 bp), PC-12 (513 bp), PC-14 (238 bp), PC-15 (241 bp), PC-16 (252 bp), and PL-2 (401 bp) were positively amplified. (B) cDNAs of the late log stage, the PC-1 (322 bp), PC-4 (162 bp), PC-5 (456 bp), PL-2 (401 bp), and PL-3 (277 bp) were obtained. (C) The cDNAs of the stationary stage, the PC-1 (322 bp), PC-2 (291 bp), PC-8 (251 bp), and PC-12 (513 bp) were successfully amplified. In all the three growth stages, the positive control of 16S rRNA gene, recA gene, and PC-3 (the positive control gene from A. tumefaciens C58) were all obtained. The samples without cDNAs (water controls) for the 16S rRNA and recA genes were all negative.