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. 2004 Jan;72(1):414–429. doi: 10.1128/IAI.72.1.414-429.2004

FIG. 9.

FIG. 9.

Semiquantitative analysis of changes in PMNL gene expression. (A) cDNAs corresponding to 1 μg of RNA extracted from uninfected PMNL or PMNL infected with C. albicans at an MOI of 0.1, 0.5, or 5 were subjected to PCR with primers specific for C. albicans actin. The amount of amplified fragment was compared to that from cDNA corresponding to 1,000, 100, 10, 1, and 0.1 ng of C. albicans RNA. (B) RT-PCR analysis visualized by Southern hybridization. Amplification was carried out for low (15 cycles for PAC1, PAI2, S28, and ACTB, 20 cycles for TNFA and TR3, 25 cycles for N.E., and 30 cycles for HNP1) or high (20 cycles for PAC1, PAI2, S28, and ACTB, 25 cycles for TNFA and TR3, 30 cycles for N.E., and 35 for HNP1) intensity. Numbers indicate the intensity of hybridization relative to that in uninfected PMNL (set to 1).