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. 2012 Sep 11;7(9):e44317. doi: 10.1371/journal.pone.0044317

Figure 4. Assessing inhibition of ricin binding by individual mAbs. (Panels A, B).

Figure 4

Biotin-labeled ricin (50 ng/ml: 770 pM) was mixed with indicated mAbs (20 µg/ml; 133 nM) and then applied to 96-well microtiter plates coated with ASF (4 µg/ml), as described in Materials and Methods. The percent binding of biotin-ricin to ASF was then detected using a standard ELISA protocol in which plates were treated with avidin-HRP and TMB substrate. Each symbol represents the average of at least three replicate wells. (Panels C, D) Differential reactivity of indicated mAbs with ricin or ricin-receptor complexes. Ninety-six well microtiter plates coated with ricin (open shapes) or ricin-ASF (closed shapes) were probed with indicated mAbs (10 µg/ml: 66.7 nM) JB4 and B/J F9 (C), and C/M A2 (D). This experiment was repeated at least 2 times.