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. 2012 Sep 11;7(9):e44317. doi: 10.1371/journal.pone.0044317

Figure 8. Differential reactivity of RTB-specific mAbs with ricin by Western blot analysis.

Figure 8

Ricin holotoxin was suspended in Laemmli sample buffer (lanes 1), suspended in Laemmli sample buffer and boiled (lanes 2), or suspended in Laemmli sample buffer containing β-mercaptoethanol and boiled (lanes 3) before being subjected to SDS-12% PAGE and Western blotting with the indicated mAbs. Panels correspond to the following mAbs: (A) C/M A2, (B) B/J F9, (C) 24B11 and (D) JB4. The arrowheads (far left) indicate the location of ricin holotoxin under non-reducing conditions (solid) and RTB (open). Each blot is representative of at least 4 independent experiments.