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. 2012 Sep 11;7(9):e44361. doi: 10.1371/journal.pone.0044361

Figure 6. Analysis of expression of MA-MADS5 protein in various regions of banana fruit tissue. a.

Figure 6

Various regions of banana fruit from the cultivar Giant governor (Musa sp, AAA group, subgroup Cavendish) have been indicated (zones A–E). b Detection of MA-MADS5 protein in A and B zones of preclimacteric (0 DAH), climacteric (88 DAH) and postclimacteric (92 DAH) banana fruit by immunoblotting using anti-MA-MADS5 polyclonal antibody (1∶1000 dilution) (upper panel). Equal amounts of small nuclear protein was loaded in each lane and shown as loading control (lower panel). c–e Immunodetection of MA-MADS5 protein levels in C, D and E zones (including both peripheral and central parts) of preclimacteric, climacteric and postclimacteric fruit pulp tissues of banana (upper panel). Equal amounts of small nuclear protein was loaded in each lane and shown as loading control (lower panels of c, d and e respectively). Representative images from at least three independent experiments are shown for Figure b–e. f and g Immunolocalization of MA-MADS5 protein in A and B zones in postclimacteric banana pulp tissues (92 DAH). The legends of the panels I–IV are similar as described in Figure.5 b–e.