Figure 6. Expression of flavonoid biosynthetic genes in developing seed.

Seeds were obtained from the yellow-seeded and black-seeded plants 10 days after pollination. The expression of the different genes was detected by quantitative Real-time PCR. Transcripts for two flavonoid EBGs BrTT6 (encode F3H, flavanone 3-hydroxylase) and BrTT7 (encode F3′H, flavanone 3′-hydroxylase), three flavonoid LBGs BrDFR (encode dihydroflavonol reductase), BrBAN (encode anthocyanidin reductase) and BrLDOX (encode LDOX, leucoanthocyanidin dioxygenase) in B. rapa. The comparative Ct method was used to calculate the levels of transcripts relative to black-seeded plant. (“B” in the legend is the black seed and “Y” is the yellow seed).