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Protein stability measured using urea and temperature melts. A. Changes in the CD signal at 222 nm, and B. changes in the protein fluorescence with excitation at 280 nm as a function of urea concentration. C. Changes in the CD signal at 222 nm, and D. changes in the protein fluorescence with excitation at 280 nm as a function of increasing solution temperature. In all the panels, black and red curves correspond to dystrophin (Dys) and utrophin (Utr) N-ABDs respectively.
