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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Jul 25;264(1):73–83. doi: 10.1016/j.taap.2012.07.019

Fig. 2.

Fig. 2

CYP4Z1 overexpression regulates VEGF-A and TIMP-2 levels in breast cancer cells. (A) mRNA expression of several major pro-and anti-angiogenic factors from T47D and BT-474 cells transfected or untransfected with CYP4Z1 were examined using quantitative real-time polymerase chain reaction (qPCR). Each data point is expressed as mean ± S.D. from 3 independent experiments (n=3). (B) T47D-CYP4Z1 cells and BT-474-CYP4Z1 cells were plated at a concentration of 1.5×105 cells per well in 6-well plates. After incubation for 24 h, the cells were washed with phosphate buffer, and then serum-starved overnight before treating with or without HET0016 (100 nM) or WIT002 (1 M) for 48 h. Vector control cells were used as negative control. The levels of VEGF-A and TIMP-2 in culture supernatants were determined using ELISA kits. Results are shown as mean ± S.D. from 3 independent experiments (n=3). *P < 0.05, **P < 0.01 vs. control group; #P < 0.05 vs. CYP4Z1 transfeciton group.