Fig. 7.

Confirmation of axonal growth from NPC transplant along the corpus callosum. In the top panel, double staining of AP and GFAP demonstrates that AP-positive fibers did not colocalize with astrocyte processes (identified by GFAP). In the middle panel, double staining of AP and Tuj1 shows many AP-positive fibers also stained by Tuj1, a specific neuronal marker. In the bottom panel, True Blue retrograde labeling of the corpus callosum colocalized with AP-positive cells in the graft, confirming that AP-positive fibers along the corpus callosum are derived from transplanted NPC neurons. Scale bar =100 μm. AP, alkaline phosphatase; GFAP, glial fibrillary acidic protein.