TABLE 1.
Effect of phenylhydrazine, hydroxylamine, and amino acids on serine dehydratase activity in cell extracts of the C. jejuni 11168 wild type and sdaA mutanta
| Addition |
l-Serine dehydratase activity (nmol min−1 mg of protein−1) for:
|
|
|---|---|---|
| 11168 wild-type | 11168 sdaA mutant | |
| None | 434 ± 42 | 40 ± 9 |
| 10 mM phenylhydrazine | 402 ± 81 | 0 |
| 1 mM hydroxylamine | 430 ± 16 | 0 |
| 1 mM isoleucine | 431 ± 62 | 0 |
| 100 mM d-serine | 260 ± 93 | ND |
| 100 mM l-threonine | 194 ± 11 | ND |
| 50 mM l-cysteine | 22 ± 9 | ND |
a
The mean serine dehydratase activity ± standard deviation is indicated for experiments performed in duplicate. ND, not determined.