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In silico mutant experiments and analysis of neuronal SM-SNARE network motif. (a) Fusion curves of five in silico experiments with different initial concentrations of the nSM protein in the mutant neuronal SSNM system which eliminates the nSM(Munc18-1) binding to closed nSyx(syntaxin-1) (Figure 2c). (b) A comparison of fusion levels between the yeast SSNM, neuronal SSNM and mutant SSNM at steady state with respect to the SM protein concentration.
