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. 2012 Jun 15;2:13. doi: 10.1186/2044-5040-2-13

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Copyright ©2012 Ippolito et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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4',6-Diamidino-2-phenylindole (DAPI) region sizes interpreted by G-Tool. (A) Histogram showing DAPI + region sizes identified by G-Tool in a dataset containing >23,000 nuclei (C57BL/6 hindlimb satellite cells). The histogram is color coded to reflect the different sizes of nuclei that are visible in the library of images. (B) Left: An example satellite cell colony. Right: G-Tool rasterized version (magnified) of this relatively simple colony. Red denotes myosin heavy chain (MHC) + cytoplasmic regions. The DAPI + regions are colored according to the scheme defined by the histogram in (A). (C) Left: An example of a very dense satellite cell colony. Right: G-Tool rasterized version (magnified). Human counting of the regions of densely packed nuclei would be highly variable.