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SP-D aggregates only acapsular C. neoformans. Cells (106) in 100 μl were incubated in PBS containing 1.5 mM calcium for 60 min at 37°C in the presence (+ SP-D) or absence (− SP-D) of 10 μg of SP-D/ml as indicated and analyzed by light microscopy (magnification, ×10). Results from an experiment representative of three independent experiments are shown.
