FIG. 3.

SP-D binds to capsule components of C. neoformans. (A) Microtiter wells were coated with increasing amounts of the indicated capsular components and incubated with SP-D (1 μg/ml). Nonspecific binding, measured in wells coated with coating buffer only, was subtracted from total binding for each data point. (B) Binding of increasing concentrations of SP-D (0 to 2,000 ng/ml) to wells coated with 500 ng of the indicated capsular component. Binding was corrected for background by subtracting nonspecific binding, as measured for each SP-D concentration in wells coated with coating buffer only, from total binding. SP-D binding was detected as described in Materials and Methods. Results shown are for an experiment representative of three independent experiments each performed in triplicate. Values are means ± standard deviations (SD) (n = 3).