FIG. 6.

SP-D-induced aggregation of acapsular C. neoformans can be inhibited by GXM, mannan, and maltose. Shown are the results of flow cytometric analysis of SP-D-induced aggregation of acapsular C. neoformans (strain B4131) incubated with SP-D (10 μg/ml) in the presence of increasing concentrations of the indicated saccharides. Scatter plots are shown in panel A, whereas for panel B aggregation was quantified by counting the number of events detected in the upper right and lower right quadrants of the scatter plots. Data are presented as percentages of maximal aggregation as measured in the absence of a saccharide inhibitor. Data represent the means ± SD of results from three independent experiments. SSC, side scatter; FSC, forward scatter.