FIG. 5.

Invasion of trophoblasts by L. monocytogenes. Monolayers of cells were grown on glass coverslips. Trophoblasts were infected with L. monocytogenes 10403S (wild type [wt]) and the InlA deletion mutant (ΔA), the InlB deletion mutant (ΔB), the InlA InlB double deletion mutant (ΔAB), the InlA complementation mutant (cA), and the InlA InlB complementation mutant (cAB). BeWo cells were infected at an MOI of 1:1 (A and B), and primary human trophoblasts were infected at an estimated MOI of 20:1 (C). In each case, gentamicin (50 μg/ml) was added 1 h after infection. At 2 h after infection, monolayers were lysed and the number of bacteria per coverslip was determined in triplicate. All bacterial numbers are the averages and standard deviations from three separate experiments.