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. 2012 Sep 12;6:64. doi: 10.3389/fncir.2012.00064

Figure 4.

Figure 4

HTC-Waves depend on frequency facilitation of mossy fiber to CA3 synaptic transmission and are rapidly boosted by caffeine and corticosterone. (A) Experimental arrangement used for the investigations shown in (C–G) (“P” stands for “pressure”). (B) Experimental protocol used for the investigations shown in (C and E–G). (C) A selective shutdown of mossy fiber synaptic transmission by a fluorescein (Fluo; 10 μM)-guided administration of the mGluR2 agonist DCG-IV (30 μM; 1 min) specifically to area CA3 abolished HTC-Waves without affecting DG activity (N = 8 slices/6 mice). (D) During low-frequency (0.05 Hz) EC/DG-input following 5 Hz EC/DG-input, the HTC reverted to its locked state regarding passages of neuronal activity (N = 6 slices/3 mice). (E,F) Caffeine (5 μM) quickly boosted HTC-Waves in a reversible manner (E, N = 8 slices/4 mice; F, N = 4 slices/3 mice). (G) Bath application of 100 nM corticosterone to slices rapidly amplified HTC-Waves (N = 7 slices/4 mice). (C,E,G) *p < 0.05 (paired t-test); **p < 0.01 (paired t-test); ***p < 0.001 (paired t-test).