FIG. 2.

Respiratory burst of normal human granulocytes in PBS-glucose (PBS-Glc) and conditioned medium (Cond.med.). Median fluorescence intensity (MdFI) was used as a measure of the respiratory burst of a cell. The median respiratory burst of a control group of samples served as a reference value, and percentages of increase versus this value were calculated. TIMP-1 at a concentration of 500 ng/ml significantly increased the metabolic activity of granulocytes (P < 0.05). The addition of conditioned medium significantly increased the metabolic activity of the cells (P < 0.05). Further addition of TIMP-1 did not have a significant additive effect. Data are presented as medians and ranges, excluding outliers, from four experiments.