FIG. 3.

Induction of IL-8 by V. cholerae strains and their isogenic hap mutants in T84 cells. V. cholerae were grown in 5 ml of LB at 37°C overnight and washed twice in sterile PBS. T84 cells were infected with V. cholerae strains at an MOI of 10 in serum-free DMEM/F-12 medium for 1 h. The medium was then replaced by serum-free DMEM/F-12 medium containing gentamicin (100 μg/ml) and incubated for 18 h. The levels of IL-8 in the supernatants were measured by ELISA.