Skip to main content
. 2004 Jan;72(1):389–397. doi: 10.1128/IAI.72.1.389-397.2004

FIG. 4.

FIG. 4.

FIG. 4.

FIG. 4.

(A) IL-8 induction by culture supernatant of CVD114 in T84 cells. An overnight culture of V. cholerae CVD114 was subcultured (at a dilution of 1:1,000) and grown in 5 ml of LB broth for 18 h at 30°C. The supernatant was diluted, and 100 μl of the supernatant dilutions was added to T84 cells in 24-well tissue culture plate. Supernatant from CVD115 was used as a control. The levels of IL-8 were determined by ELISA, and the cytotoxicity was determined by trypan blue staining. (B) T84 cells were either not treated (panel 1) or treated with undiluted (panel 2), 10-fold-diluted (panel 3), or 100-fold-diluted (panel 4) supernatant containing Hap. The arrow shows the detached cell clumps. (C) Supernatant from CVD115 stimulates IL-8 in T84 cells in a dose-dependent manner. An overnight culture of V. cholerae CVD115 was subcultured (at a dilution of 1:1,000) and grown in 5 ml of LB broth for 18 h at 30°C. The supernatant was diluted, and 100 μl of each diluted supernatant was added to T84 cells in a 24-well tissue culture plate. The levels of IL-8 were determined by ELISA.