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Stability of IL-8 inducer in CVD115 culture supernatant. T84 cells were incubated for 18 h with control LB medium, V. cholerae CVD115 supernatant, or heat-treated supernatant (30 min at 95°C) or were incubated for 2 h with 40 μg of trypsin/ml or 200 μg of proteinase K/ml (34), followed by heat treatment.
