Bacterial antigens induce TR6 mRNA expression in monocytes and MDC. Quantitative RT-PCR was conducted with RNA from cells stimulated for 18 to 20 h with bacterial antigens recognized by TLR2 (LTA, prepared from B. subtilis and used at 1 μg/ml), TLR4 (LPS, 100 ng/ml), or TLR9 (CpG-ODN 2006, 1 μg/ml). Cells were also stimulated with IFN-γ (100 U/ml), TNF-α (50 ng/ml), CD40L (3 μg/ml), and IFN-α (100 ng/ml), singly or in combination. Results for two of four donors are shown for each cell type.